The ideal candidate has hands-on experience in ion-exchange chromatography, affinity purification, and tangential flow filtration (TFF), with a strong understanding of purification principles and data analysis.
The successful candidate will contribute to the purification and characterization of viral vectors, biologics, or recombinant proteins to support process development and manufacturing activities
Key Responsibilities
• Execute purification processes including affinity chromatography (e.g., AVB, CaptureSelect), ion-exchange chromatography (AEX, CEX), and other polishing steps.
• Operate AKTA systems (Avant, Pure, or Pilot) for small- to large-scale purification runs.
• Perform TFF operations for buffer exchange and concentration of intermediate or final products.
• Support experimental design, data collection, and documentation following established procedures and GMP/GLP best practices.
• Assist in troubleshooting and optimization of purification processes.
• Analyze in-process and purified samples using relevant analytical methods (e.g., UV, SDS-PAGE, ddPCR, ELISA, or HPLC).
• Collaborate cross-functionally with upstream, analytical, and manufacturing teams.
• Maintain lab equipment, manage buffers and column inventory, and ensure compliance with safety and documentation standards.
Qualifications:
• Education: Master’s degree in Biochemistry, Chemical Engineering, Biotechnology, or related field.
• Experience: Minimum 2 years of hands-on purification experience in an academic or industry setting.
• Technical Skills:
o Strong knowledge and practical experience with ion-exchange and affinity chromatography.
o Familiarity with buffer preparation.
o Understanding of basic analytical methods for product characterization.
• Soft Skills:
o Strong attention to detail and excellent documentation habits.
o Effective communication and teamwork skills.
o Ability to manage multiple tasks and meet project timelines.
Preferred Qualifications
• Experience in AAV or other viral vector purification.
• Experience with AKTA systems and Unicorn software.
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